Sigrist H, Collioud A, Clémence JF, Gao H, Luginbühl R, Sanger M and Sundarababu G (1995) Opt. Petro M, Gemeiner P and Berek D (1994) J Chromatogr A665: 37–45 Jacobs JW and Fodor SPA (1994) Trends in Biotechnology 12: 19–26 13: 855-860īergström J, Löfas S and Johnsson B PCT patent application WO 90/05303ĭolder M, Michel H and Sigrist H (1990) J Prot Chem 9: 407–415įodor SPA, Stryer L, Winkler JL, Holmes CW and Solas CW PCT WO 92/10092 Gao H, Kislig E, Oranth N and Sigrist H (1994) Biotech Appl Biochem 20: 251–263 This process is experimental and the keywords may be updated as the learning algorithm improves.īarié N, Rapp M, Sigrist H and Ache HJ (1998) Biosensors and Bioelectronics. These keywords were added by machine and not by the authors. The construction of biochip microarrays was achieved by combining ink-jet printing and photobonding technologies. The described photobonding process combines several unique properties: local addressability, light-dependent covalent bonding, single-step multicompent immobilization and chemical multi-functionality through modification of the parent molecules. Neither reactive functional groups on the biomolecule nor on the material surface were required to achieve single step probe molecule immobilization. Photolinker polymer mediated covalent immobilization of bio-molecules was attained under mild and non-invasive conditions. The product, OptoDex, served as the basis for the syntheseis of several photoactivatable OptoDex analogues. In this study dextran was modified with photoactivatable groups. In biochip technology, hydrophilic polysaccharides (or bovine serum albumin) are appreciated as molecular barriers due to their biological inertness and biocompatibility. Generated intermediates bond to all neighboring materials that are either substrate or biomolecule. When irradiated with light, the photoactivatable chemical groups turn into reactive intermediates. We have designed and synthesised photolinker polymers carrying several photoactivatable chemical functional groups (e.g. Photobonding is a unique procedure for bioprobe immobilization on “inert” surfaces by a light-induced process. Immobilization of biomolecules on material surfaces while retaining their biological functions is a major challenge in biochip manufacturing.
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